BMRB Entry 7429
BMRB Entry DOI: doi:10.13018/BMR7429
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Title: Backbone 1H and 15N Chemical Shift Assignments for the Phosphotyrosine Binding Domain of Insulin Receptor Substrate 1 in the Apo and Phosphopeptide Bound Forms PubMed: 19053277
Deposition date: 2008-09-18 Original release date: 2008-10-03
Authors: Stone, Martin; Jarymowycz, Virginia
Citation: Stone, Martin; Jarymowycz, Virginia. "Remote Changes in Dynamics of the Phosphotyrosine-Binding Domain of Insulin Receptor Substrate-1 Induced by Phosphopeptide Binding" Biochemistry 47, 13371-13382 (2008).
Assembly members:
Phosphotyrosine-Binding_Domain_of_Insulin_Receptor_Substrate-1, polymer, 111 residues, Formula weight is not available
Interleukin-4_Receptor_Phosphopeptide, polymer, 111 residues, 261.168 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis Host organism: Escherichia coli Vector: pET30a
Entity Sequences (FASTA):
Phosphotyrosine-Binding_Domain_of_Insulin_Receptor_Substrate-1: GPAFKEVWQVILKPKGLGQT
KNLIGIYRLCLTSKTISFVK
LNSEAAAVVLQLMNIRRCGH
SENFFFIEVGRSAVTGPGEF
WMQVDDSVVAQNMHETILEA
MRAMSDEFRPR
Interleukin-4_Receptor_Phosphopeptide: LVIAGNPAXRS
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 98 |
| 1H chemical shifts | 98 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Monomer | 1 |
| 2 | Peptide | 2 |
Entities:
Entity 1, Monomer 111 residues - Formula weight is not available
| 1 | GLY | PRO | ALA | PHE | LYS | GLU | VAL | TRP | GLN | VAL | ||||
| 2 | ILE | LEU | LYS | PRO | LYS | GLY | LEU | GLY | GLN | THR | ||||
| 3 | LYS | ASN | LEU | ILE | GLY | ILE | TYR | ARG | LEU | CYS | ||||
| 4 | LEU | THR | SER | LYS | THR | ILE | SER | PHE | VAL | LYS | ||||
| 5 | LEU | ASN | SER | GLU | ALA | ALA | ALA | VAL | VAL | LEU | ||||
| 6 | GLN | LEU | MET | ASN | ILE | ARG | ARG | CYS | GLY | HIS | ||||
| 7 | SER | GLU | ASN | PHE | PHE | PHE | ILE | GLU | VAL | GLY | ||||
| 8 | ARG | SER | ALA | VAL | THR | GLY | PRO | GLY | GLU | PHE | ||||
| 9 | TRP | MET | GLN | VAL | ASP | ASP | SER | VAL | VAL | ALA | ||||
| 10 | GLN | ASN | MET | HIS | GLU | THR | ILE | LEU | GLU | ALA | ||||
| 11 | MET | ARG | ALA | MET | SER | ASP | GLU | PHE | ARG | PRO | ||||
| 12 | ARG |
Entity 2, Peptide 111 residues - 261.168 Da.
| 1 | LEU | VAL | ILE | ALA | GLY | ASN | PRO | ALA | PTR | ARG | ||||
| 2 | SER |
Samples:
PTB_Domain_of_IRS-1_Bound_State: Phosphotyrosine-Binding Domain of Insulin Receptor Substrate-1, [U-15N], 200 uM
PTB_Domain_of_IRS-1_Bound_Sample_Conditions: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | PTB_Domain_of_IRS-1_Bound_State | isotropic | PTB_Domain_of_IRS-1_Bound_Sample_Conditions |
| 3D CBCA(CO)NH | PTB_Domain_of_IRS-1_Bound_State | isotropic | PTB_Domain_of_IRS-1_Bound_Sample_Conditions |
Software:
FELIX, Accelrys Software Inc. - processing
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 15959 |
| PDB | |
| DBJ | BAA11026 BAC32308 BAE24991 BAG10207 |
| EMBL | CAA41264 CAA49378 |
| GB | AAA39335 AAB21608 AAB27175 AAH53895 AAI56077 |
| PRF | 1712323A |
| REF | NP_005535 NP_034700 NP_037101 XP_001109882 XP_001134743 |
| SP | P35568 P35569 P35570 Q28224 |
| AlphaFold | P35570 Q28224 P35568 P35569 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts