BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16753

Title: NMR Study on the Structural Changes of Cytochrome P450cam upon the Complex Formation with Putidaredoxin   PubMed: 12842870

Deposition date: 2010-03-03 Original release date: 2010-03-24

Authors: Tosha, Takehiko; Yoshioka, Shiro; Takahashi, Satoshi; Ishimori, Koichiro; Shimada, Hideo; Morishima, Isao

Citation: Tosha, Takehiko; Yoshioka, Shiro; Takahashi, Satoshi; Ishimori, Koichiro; Shimada, Hideo; Morishima, Isao. "NMR Study on the Structural Changes of Cytochrome P450cam on the Complex Formation with Putidaredoxin"  J. Biol. Chem. 278, 39809-39821 (2003).

Assembly members:
P450cam, polymer, 414 residues, Formula weight is not available
Pdx, polymer, 106 residues, Formula weight is not available

Natural source:   Common Name: Pseudomonas putida   Taxonomy ID: 303   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas putida

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: na

Entity Sequences (FASTA):
P450cam: TTETIQSNANLAPLPPHVPE HLVFDFDMYNPSNLSAGVQE AWAVLQESNVPDLVWTRCNG GHWIATRGQLIREAYEDYRH FSSECPFIPREAGEAYDFIP TSMDPPEQRQFRALANQVVG MPVVDKLENRIQELACSLIE SLRPQGQCNFTEDYAEPFPI RIFMLLAGLPEEDIPHLKYL TDQMTRPDGSMTFAEAKEAL YDYLIPIIEQRRQKPGTDAI SIVANGQVNGRPITSDEAKR MCGLLLVGGLDTVVNFLSFS MEFLAKSPEHRQELIQRPER IPAACEELLRRFSLVADGRI LTSDYEFHGVQLKKGDQILL PQMLSGLDERENACPMHVDF SRQKVSHTTFGHGSHLCLGQ HLARREIIVTLKEWLTRIPD FSIAPGAQIQHKSGIVSGVQ ALPLVWDPATTKAV
Pdx: SKVVYVSHDGTRRQLDVADG VSLMQAAVSNGIYDIVGDCG GSASCATCHVYVNEAFTDKV PAANEREIGMLECVTAELKP NSRLCCQIIMTPELDGIVVD VPDRQW

Data sets:
  • binding_constants
Data typeCount
binding constants4

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1P450cam1
2Pdx2

Entities:

Entity 1, P450cam 414 residues - Formula weight is not available

1   THRTHRGLUTHRILEGLNSERASNALAASN
2   LEUALAPROLEUPROPROHISVALPROGLU
3   HISLEUVALPHEASPPHEASPMETTYRASN
4   PROSERASNLEUSERALAGLYVALGLNGLU
5   ALATRPALAVALLEUGLNGLUSERASNVAL
6   PROASPLEUVALTRPTHRARGCYSASNGLY
7   GLYHISTRPILEALATHRARGGLYGLNLEU
8   ILEARGGLUALATYRGLUASPTYRARGHIS
9   PHESERSERGLUCYSPROPHEILEPROARG
10   GLUALAGLYGLUALATYRASPPHEILEPRO
11   THRSERMETASPPROPROGLUGLNARGGLN
12   PHEARGALALEUALAASNGLNVALVALGLY
13   METPROVALVALASPLYSLEUGLUASNARG
14   ILEGLNGLULEUALACYSSERLEUILEGLU
15   SERLEUARGPROGLNGLYGLNCYSASNPHE
16   THRGLUASPTYRALAGLUPROPHEPROILE
17   ARGILEPHEMETLEULEUALAGLYLEUPRO
18   GLUGLUASPILEPROHISLEULYSTYRLEU
19   THRASPGLNMETTHRARGPROASPGLYSER
20   METTHRPHEALAGLUALALYSGLUALALEU
21   TYRASPTYRLEUILEPROILEILEGLUGLN
22   ARGARGGLNLYSPROGLYTHRASPALAILE
23   SERILEVALALAASNGLYGLNVALASNGLY
24   ARGPROILETHRSERASPGLUALALYSARG
25   METCYSGLYLEULEULEUVALGLYGLYLEU
26   ASPTHRVALVALASNPHELEUSERPHESER
27   METGLUPHELEUALALYSSERPROGLUHIS
28   ARGGLNGLULEUILEGLNARGPROGLUARG
29   ILEPROALAALACYSGLUGLULEULEUARG
30   ARGPHESERLEUVALALAASPGLYARGILE
31   LEUTHRSERASPTYRGLUPHEHISGLYVAL
32   GLNLEULYSLYSGLYASPGLNILELEULEU
33   PROGLNMETLEUSERGLYLEUASPGLUARG
34   GLUASNALACYSPROMETHISVALASPPHE
35   SERARGGLNLYSVALSERHISTHRTHRPHE
36   GLYHISGLYSERHISLEUCYSLEUGLYGLN
37   HISLEUALAARGARGGLUILEILEVALTHR
38   LEULYSGLUTRPLEUTHRARGILEPROASP
39   PHESERILEALAPROGLYALAGLNILEGLN
40   HISLYSSERGLYILEVALSERGLYVALGLN
41   ALALEUPROLEUVALTRPASPPROALATHR
42   THRLYSALAVAL

Entity 2, Pdx 106 residues - Formula weight is not available

106 residues of oxidized form given

1   SERLYSVALVALTYRVALSERHISASPGLY
2   THRARGARGGLNLEUASPVALALAASPGLY
3   VALSERLEUMETGLNALAALAVALSERASN
4   GLYILETYRASPILEVALGLYASPCYSGLY
5   GLYSERALASERCYSALATHRCYSHISVAL
6   TYRVALASNGLUALAPHETHRASPLYSVAL
7   PROALAALAASNGLUARGGLUILEGLYMET
8   LEUGLUCYSVALTHRALAGLULEULYSPRO
9   ASNSERARGLEUCYSCYSGLNILEILEMET
10   THRPROGLULEUASPGLYILEVALVALASP
11   VALPROASPARGGLNTRP

Samples:

sample_1: P450cam 0.2 mM; potassium phosphate 50 mM; potassium chloride 50 mM; d-camphor 1 mM; Pdx0.1 – 0.8 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
1H NMRsample_1isotropicsample_conditions_1

Software:

InsightII, Accelrys Software Inc. - model drawing

NMR spectrometers:

  • Bruker Avance DRX 500 MHz
  • Bruker Avance DRX 600 MHz

Related Database Links:

BMRB 17415 19038 19740 19763 1551 19038 2278 4149 4154
PDB
DBJ BAN13286 BAA00414 BAN13288
GB AAA25760 AAA25759
REF WP_032492633 YP_009083112 WP_032492635 YP_009083114
SP P00183 P00259
AlphaFold P00183 P00259