BMRB Entry 16759
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16759
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Citation: Osheroff, Neil; Brautigan, David; Margoliash, E.. "Mapping of anion binding sites on cytochrome c by differential chemical modification of lysine residues" Proc. Natl. Acad. Sci. U.S.A. 77, 4439-4443 (1980).
PubMed: 6254024
Assembly members:
cytochrome_c, polymer, 104 residues, Formula weight is not available
PO4, non-polymer, 94.971 Da.
Natural source: Common Name: Horse Taxonomy ID: 9796 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Equus caballus
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
cytochrome_c: GDVEKGKKIFVQKCAQCHTV
EKGGKHKTGPNLHGLFGRKT
GQAPGFTYTDANKNKGITWK
EETLMEYLENPKKYIPGTKM
IFAGIKKKTEREDLIAYLKK
ATNE
- binding constants
| Data type | Count |
| binding constants | 2 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cytochrome c | 1 |
| 2 | PO4 | 2 |
Entities:
Entity 1, cytochrome c 104 residues - Formula weight is not available
| 1 | GLY | ASP | VAL | GLU | LYS | GLY | LYS | LYS | ILE | PHE | ||||
| 2 | VAL | GLN | LYS | CYS | ALA | GLN | CYS | HIS | THR | VAL | ||||
| 3 | GLU | LYS | GLY | GLY | LYS | HIS | LYS | THR | GLY | PRO | ||||
| 4 | ASN | LEU | HIS | GLY | LEU | PHE | GLY | ARG | LYS | THR | ||||
| 5 | GLY | GLN | ALA | PRO | GLY | PHE | THR | TYR | THR | ASP | ||||
| 6 | ALA | ASN | LYS | ASN | LYS | GLY | ILE | THR | TRP | LYS | ||||
| 7 | GLU | GLU | THR | LEU | MET | GLU | TYR | LEU | GLU | ASN | ||||
| 8 | PRO | LYS | LYS | TYR | ILE | PRO | GLY | THR | LYS | MET | ||||
| 9 | ILE | PHE | ALA | GLY | ILE | LYS | LYS | LYS | THR | GLU | ||||
| 10 | ARG | GLU | ASP | LEU | ILE | ALA | TYR | LEU | LYS | LYS | ||||
| 11 | ALA | THR | ASN | GLU |
Entity 2, PO4 - O4 P - 94.971 Da.
| 1 | PO4 |
Samples:
sample_1: PO4 8 mM; cytochrome c 10 mM; citrate2 – 32 mM; EDTA 0.2 mM; H2O 90%; D2O 10%
sample_2: PO4 8 mM; cytochrome c 10 mM; Cl2 – 32 mM; EDTA 0.2 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 7.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 31P NMR | sample_1 | isotropic | sample_conditions_1 |
| 31P NMR | sample_2 | isotropic | sample_conditions_1 |
Software:
not_given, not given - not given
NMR spectrometers:
- JEOL FX-90Q 90 MHz