BMRB Entry 17847

Title:
Backbone 1H, 13C, and 15N chemical shift assignments of oxidized horse heart cytochrome c
Deposition date:
2011-08-09
Original release date:
2012-03-14
Authors:
Volkov, Alexander; Van de Water, Karen; Vanwetswinkel, Sophie; van Nuland, Nico
Citation:

Citation: Volkov, Alexander; Vanwetswinkel, Sophie; Van de Water, Karen; van Nuland, Nico. "Redox-dependent conformational changes in eukaryotic cytochromes revealed by paramagnetic NMR spectroscopy"  J. Biomol. NMR 52, 245-256 (2012).
PubMed: 22318343

Assembly members:

Assembly members:
hCc, polymer, 104 residues, Formula weight is not available
HEC, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: horse   Taxonomy ID: 9796   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Equus caballus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pUCcc

Data sets:
Data typeCount
13C chemical shifts298
15N chemical shifts208
1H chemical shifts224

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hCc1
2HEC2

Entities:

Entity 1, hCc 104 residues - Formula weight is not available

1   GLYASPVALGLULYSGLYLYSLYSILEPHE
2   VALGLNLYSCYSALAGLNCYSHISTHRVAL
3   GLULYSGLYGLYLYSHISLYSTHRGLYPRO
4   ASNLEUHISGLYLEUPHEGLYARGLYSTHR
5   GLYGLNALAPROGLYPHETHRTYRTHRASP
6   ALAASNLYSASNLYSGLYILETHRTRPLYS
7   GLUGLUTHRLEUMETGLUTYRLEUGLUASN
8   PROLYSLYSTYRILEPROGLYTHRLYSMET
9   ILEPHEALAGLYILELYSLYSLYSTHRGLU
10   ARGGLUASPLEUILEALATYRLEULYSLYS
11   ALATHRASNGLU

Entity 2, HEC - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_1: hCc, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; H2O 95%; D2O 5%

sample_2: hCc, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 115 mM; pH: 6.0; pressure: 1 atm; temperature: 303 K

sample_conditions_2: ionic strength: 15 mM; pH: 6.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_2
3D NOESY-HSQCsample_2isotropicsample_conditions_2

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Varian Uniform NMR System 800 MHz
  • Varian Uniform NMR System 600 MHz

Related Database Links:

BMRB 1058 1107 1108 1109 1110 1111 1112 1113 1114 1116 1170 1171 1404 16759 17120 17340 1736 1783 17848 1785 1787 1789 216 220 224 2366 2367 2368 243 244 25640 274 285 286 316 317 336 4189 436 437 438 439
PDB
GB AAB33495 AAI05398 AAX77008 ABA06541 AEB61027
PRF 610169A 711086A
REF NP_001039526 NP_001123442 NP_001157486 XP_004007999 XP_004418964
SP P00004 P62894 P62895 P62896 P68096
TPG DAA30512
AlphaFold P00004 P62894 P62895 P62896 P68096

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks