Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17847
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Citation: Volkov, Alexander; Vanwetswinkel, Sophie; Van de Water, Karen; van Nuland, Nico. "Redox-dependent conformational changes in eukaryotic cytochromes revealed by paramagnetic NMR spectroscopy" J. Biomol. NMR 52, 245-256 (2012).
PubMed: 22318343
Assembly members:
hCc, polymer, 104 residues, Formula weight is not available
HEC, non-polymer, 618.503 Da.
Natural source: Common Name: horse Taxonomy ID: 9796 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Equus caballus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pUCcc
Entity Sequences (FASTA):
hCc: GDVEKGKKIFVQKCAQCHTV
EKGGKHKTGPNLHGLFGRKT
GQAPGFTYTDANKNKGITWK
EETLMEYLENPKKYIPGTKM
IFAGIKKKTEREDLIAYLKK
ATNE
Data type | Count |
13C chemical shifts | 298 |
15N chemical shifts | 208 |
1H chemical shifts | 224 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | hCc | 1 |
2 | HEC | 2 |
Entity 1, hCc 104 residues - Formula weight is not available
1 | GLY | ASP | VAL | GLU | LYS | GLY | LYS | LYS | ILE | PHE | ||||
2 | VAL | GLN | LYS | CYS | ALA | GLN | CYS | HIS | THR | VAL | ||||
3 | GLU | LYS | GLY | GLY | LYS | HIS | LYS | THR | GLY | PRO | ||||
4 | ASN | LEU | HIS | GLY | LEU | PHE | GLY | ARG | LYS | THR | ||||
5 | GLY | GLN | ALA | PRO | GLY | PHE | THR | TYR | THR | ASP | ||||
6 | ALA | ASN | LYS | ASN | LYS | GLY | ILE | THR | TRP | LYS | ||||
7 | GLU | GLU | THR | LEU | MET | GLU | TYR | LEU | GLU | ASN | ||||
8 | PRO | LYS | LYS | TYR | ILE | PRO | GLY | THR | LYS | MET | ||||
9 | ILE | PHE | ALA | GLY | ILE | LYS | LYS | LYS | THR | GLU | ||||
10 | ARG | GLU | ASP | LEU | ILE | ALA | TYR | LEU | LYS | LYS | ||||
11 | ALA | THR | ASN | GLU |
Entity 2, HEC - C34 H34 Fe N4 O4 - 618.503 Da.
1 | HEC |
sample_1: hCc, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; H2O 95%; D2O 5%
sample_2: hCc, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 115 mM; pH: 6.0; pressure: 1 atm; temperature: 303 K
sample_conditions_2: ionic strength: 15 mM; pH: 6.0; pressure: 1 atm; temperature: 303 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_2 |
3D NOESY-HSQC | sample_2 | isotropic | sample_conditions_2 |
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
Analysis, CCPN - chemical shift assignment
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks