BMRB Entry 17120
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17120
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Citation: Shao, Weiping; Liu, Gaohua; Tang, Wenxia. "Binding of 1-methylimidazole to cytochrome c: kinetic analysis and resonance assignments by two-dimensional NMR" Biochim. Biophys. Acta 1248, 177-185 (1995).
PubMed: 7748900
Assembly members:
cytc, polymer, 105 residues, Formula weight is not available
1MZ, non-polymer, 83.112 Da.
Natural source: Common Name: Horse Taxonomy ID: 9795 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Equus caballus
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
cytc: MGDVEKGKKIFVQKCAQCHT
VEKGGKHKTGPNLHGLFGRK
TGQAPGFSYTDANKNKGITW
KEETLMEYLENPKKYIPGTK
MIFAGIKKKTEREDLIAYLK
KATNE
- chemical rates
| Data type | Count |
| binding constants | 5 |
| kinetic rates | 4 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cytc | 1 |
| 2 | 1MZ | 2 |
Entities:
Entity 1, cytc 105 residues - Formula weight is not available
| 1 | MET | GLY | ASP | VAL | GLU | LYS | GLY | LYS | LYS | ILE | ||||
| 2 | PHE | VAL | GLN | LYS | CYS | ALA | GLN | CYS | HIS | THR | ||||
| 3 | VAL | GLU | LYS | GLY | GLY | LYS | HIS | LYS | THR | GLY | ||||
| 4 | PRO | ASN | LEU | HIS | GLY | LEU | PHE | GLY | ARG | LYS | ||||
| 5 | THR | GLY | GLN | ALA | PRO | GLY | PHE | SER | TYR | THR | ||||
| 6 | ASP | ALA | ASN | LYS | ASN | LYS | GLY | ILE | THR | TRP | ||||
| 7 | LYS | GLU | GLU | THR | LEU | MET | GLU | TYR | LEU | GLU | ||||
| 8 | ASN | PRO | LYS | LYS | TYR | ILE | PRO | GLY | THR | LYS | ||||
| 9 | MET | ILE | PHE | ALA | GLY | ILE | LYS | LYS | LYS | THR | ||||
| 10 | GLU | ARG | GLU | ASP | LEU | ILE | ALA | TYR | LEU | LYS | ||||
| 11 | LYS | ALA | THR | ASN | GLU |
Entity 2, 1MZ - C4 H7 N2 - 83.112 Da.
| 1 | 1MZ |
Samples:
sample_1: cytc 8 mM; 1MZ 40 mM; D2O 100%
sample_conditions_1: pH: 7.0; pressure: ambient atm; temperature: 319 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D EXSY | sample_1 | isotropic | sample_conditions_1 |
Software:
software, na - na
NMR spectrometers:
- Bruker AM 500 MHz