BMRB Entry 17215
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17215
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Citation: Rodriguez-Maranon, Maria; Qiu, Feng; Stark, Ruth; White, Steven; Zhang, Xuejun; Foundling, Stephen; Rodriguez, Veronica; Schilling, Curtis; Bunce, Richard; Rivera, Mario. "13C NMR Spectroscopic and X-ray Crystallographic Study of the Role Played by Mitochondrial Cytochrome b5 Heme Propionates in the Electrostatic Binding to Cytochrome c" Biochemistry 35, 16378-16390 (1996).
PubMed: 8973214
Assembly members:
OMcb5, polymer, 146 residues, Formula weight is not available
Cc, polymer, 105 residues, Formula weight is not available
Natural source: Common Name: Horse Taxonomy ID: 9796 Superkingdom: Eukaryota Kingdom: Metaoza Genus/species: Equus caballus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: MRL2
Entity Sequences (FASTA):
OMcb5: MAAVDASGSNGKGQGVETSV
TYYRLEEVAKRNSSKEIWLV
IHGRVYDVTRFLNEHPGGEE
VLLEQAGADASESFEDVGHS
SDAREMLKQYYIGDVHPNDL
KPEGGGKDPSKNNTCKSCWI
YWLLPVVGAVLLGFLCRYYT
SESTSS
Cc: MGDVEKGKKIFVQKCAQCHT
VEKGGKHKTGPNLHGLFGRK
TGQAPGFSYTDANKNKGITW
KEETLMEYLENPKKYIPGTK
MIFAGIKKKTEREDLIAYLK
KATNE
- binding_constants
| Data type | Count |
| binding constants | 1 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | OMcb5 | 1 |
| 2 | Cc | 2 |
Entities:
Entity 1, OMcb5 146 residues - Formula weight is not available
| 1 | MET | ALA | ALA | VAL | ASP | ALA | SER | GLY | SER | ASN | ||||
| 2 | GLY | LYS | GLY | GLN | GLY | VAL | GLU | THR | SER | VAL | ||||
| 3 | THR | TYR | TYR | ARG | LEU | GLU | GLU | VAL | ALA | LYS | ||||
| 4 | ARG | ASN | SER | SER | LYS | GLU | ILE | TRP | LEU | VAL | ||||
| 5 | ILE | HIS | GLY | ARG | VAL | TYR | ASP | VAL | THR | ARG | ||||
| 6 | PHE | LEU | ASN | GLU | HIS | PRO | GLY | GLY | GLU | GLU | ||||
| 7 | VAL | LEU | LEU | GLU | GLN | ALA | GLY | ALA | ASP | ALA | ||||
| 8 | SER | GLU | SER | PHE | GLU | ASP | VAL | GLY | HIS | SER | ||||
| 9 | SER | ASP | ALA | ARG | GLU | MET | LEU | LYS | GLN | TYR | ||||
| 10 | TYR | ILE | GLY | ASP | VAL | HIS | PRO | ASN | ASP | LEU | ||||
| 11 | LYS | PRO | GLU | GLY | GLY | GLY | LYS | ASP | PRO | SER | ||||
| 12 | LYS | ASN | ASN | THR | CYS | LYS | SER | CYS | TRP | ILE | ||||
| 13 | TYR | TRP | LEU | LEU | PRO | VAL | VAL | GLY | ALA | VAL | ||||
| 14 | LEU | LEU | GLY | PHE | LEU | CYS | ARG | TYR | TYR | THR | ||||
| 15 | SER | GLU | SER | THR | SER | SER |
Entity 2, Cc 105 residues - Formula weight is not available
| 1 | MET | GLY | ASP | VAL | GLU | LYS | GLY | LYS | LYS | ILE | ||||
| 2 | PHE | VAL | GLN | LYS | CYS | ALA | GLN | CYS | HIS | THR | ||||
| 3 | VAL | GLU | LYS | GLY | GLY | LYS | HIS | LYS | THR | GLY | ||||
| 4 | PRO | ASN | LEU | HIS | GLY | LEU | PHE | GLY | ARG | LYS | ||||
| 5 | THR | GLY | GLN | ALA | PRO | GLY | PHE | SER | TYR | THR | ||||
| 6 | ASP | ALA | ASN | LYS | ASN | LYS | GLY | ILE | THR | TRP | ||||
| 7 | LYS | GLU | GLU | THR | LEU | MET | GLU | TYR | LEU | GLU | ||||
| 8 | ASN | PRO | LYS | LYS | TYR | ILE | PRO | GLY | THR | LYS | ||||
| 9 | MET | ILE | PHE | ALA | GLY | ILE | LYS | LYS | LYS | THR | ||||
| 10 | GLU | ARG | GLU | ASP | LEU | ILE | ALA | TYR | LEU | LYS | ||||
| 11 | LYS | ALA | THR | ASN | GLU |
Samples:
sample_1: OMcb5, [U-13C], 1 – 2 mM; perdeuterated phosphate buffer 5 mM; Cc0 – 2.5 mM; D2O 100%
sample_conditions_1: pH: 7.0; pressure: ambient atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 13C NMR | sample_1 | isotropic | sample_conditions_1 |
Software:
software, na - na
NMR spectrometers:
- Varian XL 400 MHz